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Assay Range | 62.5--4000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Mouse IL-31 standard: 2 vials
2. One 96-well plate precoated with anti- Mouse IL-31 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Interleukin-31 (IL-31) is a cytokine belonging to the IL-6 family. The mouse IL-31 is synthesized as a 163 amino acid (aa) precursor composed of a 23 aa signal peptide and a 140 aa mature protein. Mature mouse IL-31 shares 29% and 63% aa sequence identity with human and rat IL-31, respectively. Mouse and human IL-31 show no spices cross-reactivity. IL-31 is preferentially expressed by Th2 cells. IL-31 elicits its effects by interacting with a heterodimeric receptor complex of a gp130 related molecule termed IL-31RA/GPL/GLMR and the oncostatin M receptor (OSM Rβ). In the complex, IL-31 directly binds to GPL, not OSM Rβ. Multiple isoforms of IL-31RA have been described, while only the isoform containing the entire cytoplasmic domain is capable of signaling. IL-31-mediated signaling pathways include the Jak/STAT pathway, the PI3 kinase/AKT cascade, and the MAP kinase pathway. The IL-31 receptor complex is constitutively expressed by keratinocytes and upregulated by IFNγ on monocytes. The biological activities of IL-31 are mostly unknown. It may play a role in inflammation of the skin. In transgenic mice, IL-31 is shown to be linked to the pruritis (itching) associated with nonatopic dermatitis.