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Influenza A H4N6 HA ELISA Kit ( A/mallard/Ohio/657/2002 )

NS-E10156

$599.00

Data sheet

Assay Range 109.38 - 7000 pg/ml

More info

Sensitivity
100 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant H4N6 HA standard: 10 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-H4N6 HA Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-H4N6 HA Ab: 1 vial, dilution 1:400.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

Influenza (flu) is important respiratory pathogens in mammals and can also produce highly fatal systemic disease in domestic poultry. Influenza A is found in a wide variety of bird and mammal species and is further divided into subtypes based on differences in the membrane proteins hemagglutinin (HA) and neuraminidase (NA). H4N6 is a subtype of Influenza A which was first isolated from pigs with pneumonia on a commercial swine farm in Canada. Hemagglutinin (HA) is a single-pass type I integral membrane glycoprotein from the influenza virus, and comprises over 80% of the envelope proteins present in the virus particle. HA plays a major role in the determination of host range restriction and virulence.

Standard Curve

Citations:

1)Self-assembled star-shaped chiroplasmonic gold nanoparticles for an ultrasensitive chiro-immunosensor for viruses, Syed Rahin Ahmed, et al, RSC Advances, 2017,7, 40849-40857

2) Direct immunosensing of avian influenza A virus in whole blood using hybrid nanocomposites
John Buozis, Syed Rahin Ahmed, Rohit Chand, Éva Nagy, Suresh Neethirajan
bioRxiv 343376; doi: https://doi.org/10.1101/343376


3) Chiral MoS2 Quantum Dots: Dual‐Mode Detection Approaches for Avian Influenza Viruses, Syed Rahin Ahmed Suresh Neethirajan, Global Challenges, Volume2, Issue4 April 26, 2018 1700071




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