More info
Assay Range | 0.312-20 ng/ml |
Sensitivity | 0.1 ng/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Sample Type | Cell culture supernatants. |
Detection Method | Chromogenic |
Kit Components
1. Recombinant EquineIL-1 ra/IL-1F3 standard: 2 vials
2. One 96-well plate coated with Equine IL-1 ra/IL-1F3 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer:12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9. Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Background
Interleukin-1 receptor antagonist (IL-1ra), also known as IL-1F3, ICIL-1RA, IL-1 inhibitor, is a member of the IL-1 family. Human IL-1ra is synthesized as a 177 aa precursor protein composed of a 25 aa signal sequence and a 152 aa mature region. Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and shows species cross-reactivity on mouse cells. It is reported that IL-1ra is expressed on diverse cells including monocytes, Sertoli cells, hepatocytes, adipocytes, synovial fibroblasts, mast cells, pancreatic β-cells, and intestinal epithelial cells.
IL-1 initiates a series of biological processes by binding to widely expressed IL-1 type I receptors (IL-1 RI). On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, leading to reduced collagen synthesis and chondrocyte apoptosis. IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations. IL-1ra blocks IL-1 action through competitive inhibition. Although IL-1ra fills the IL-1 binding site in IL-1 RI, it is unable to assist the formation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. In other words, IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity.