More info
Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| EPH receptor B3 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | IHC-P, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Eph receptor B3(982-998aa SIQDMRLQMNQTLPVQV), identical to the related rat and mouse sequences. | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | EPHB3 |
Protein | Ephrin type-B receptor 3 |
Uniprot ID | P54753 |
Function | Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Generally has an overlapping and redundant function with EPHB2. Like EPHB2, functions in axon guidance during development regulating for instance the neurons forming the corpus callosum and the anterior commissure, 2 major interhemispheric connections between the temporal lobes of the cerebral cortex. In addition to its role in axon guidance plays also an important redundant role with other ephrin-B receptors in development and maturation of dendritic spines and the formation of excitatory synapses. Controls other aspects of development through regulation of cell migration and positioning. This includes angiogenesis, palate development and thymic epithelium development for instance. Forward and reverse signaling through the EFNB2/EPHB3 complex also regulate migration and adhesion of cells that tubularize the urethra and septate the cloaca. Finally, plays an important role in intestinal epithelium differentiation segregating progenitor from differentiated cells in the crypt. |
Tissue Specificity | Ubiquitous. |
Sub-cellular localization | Cell membrane. |
Sequence Similarities | Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily. |
Aliases | Cek10 antibody|EK2 antibody|Embryonic kinase 2 antibody|EPH Like Tyrosine Kinase 2 antibody|EPH-like kinase 2 antibody|EphB3 antibody|EPHB3_HUMAN antibody|Ephrin receptor EphB3 antibody|Ephrin type B receptor 3 antibody|Ephrin type-B receptor 3 antibody|ETK2 antibody|HEK2 antibody|Human Embryo Kinase 2 antibody|Mdk5 antibody|Sek4 antibody|TYRO6 antibody|Tyrosine protein kinase receptor HEK2 antibody|Tyrosine protein kinase TYRO6 antibody|Tyrosine-protein kinase TYRO6 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Rat Mouse | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human, Rat Mouse | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Eph receptor B3 antibody, ASA-B0659, Western blotting
Lane 1: HELA Cell Lysate
Lane 2: A549 Cell Lysate
Lane 1: HELA Cell Lysate
Lane 2: A549 Cell Lysate
Anti-Eph receptor B3 antibody, ASA-B0659, IHC(P)
IHC(P): Human Lung Cancer Tissue
IHC(P): Human Lung Cancer Tissue
