Cytochrome P450 2E1 Antibody

Overview

Long Name	Antibody Type	Antibody Isotype	Host	Species Reactivity	Validated Applications	Purification
cytochrome P450, family 2, subfamily E, polypeptide 1	Polyclonal	IgG	Rabbit	Mouse, Rat	IHC-P, WB	Immunogen affinity purified.
Immunogen
A synthetic peptide corresponding to a sequence at the N-terminus of mouse Cytochrome P450 2E1(94-112aa KNEFSGRGDIPVFQEYKNK), identical to the related rat sequence.

Properties

Form	Lyophilized
Size	100 µg/vial
Contents	Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request.
Concentration	Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration).
Storage	At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing.

Additional Information Regarding the Antigen

Gene	CYP2E1
Protein	Cytochrome P450 2E1
Uniprot ID	Q05421
Function	Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites.
Tissue Specificity	Highest level in the liver and to a lesser extent in the kidney, with a higher level in the male kidney than in the female.
Sub-cellular localization	Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
Sequence Similarities
Aliases	4-nitrophenol 2-hydroxylase antibody|CP2E1_HUMAN antibody|CPE 1 antibody|CPE1 antibody|CYP2E antibody|CYP2E1 antibody|CYPIIE1 antibody|Cytochrome P450 2E1 antibody|Cytochrome P450 family 2 subfamily E polypeptide 1 antibody|Cytochrome P450 isozyme 3A antibody|Cytochrome P450 LM3A antibody|Cytochrome P450 subfamily IIE(ethanol inducible) polypeptide 1 antibody|Cytochrome P450 subfamily IIE polypeptide 1 antibody|Cytochrome P450, family 2, subfamily e, polypeptide 1 antibody|cytochrome P450, subfamily IIE(ethanol-inducible), polypeptide 1 antibody|Cytochrome P450, subfamily IIE antibody|Cytochrome P450-ALC antibody|Cytochrome P450-J antibody|Cytochrome P450RLM6 antibody|EC 1.14.13.- antibody|EC 1.14.13.n7 antibody|Ethanol-inducible P450 antibody|Flavoprotein linked monooxygenase antibody|Flavoprotein-linked monooxygenase antibody|MGC133529 antibody|Microsomal monooxygenase antibody|OTTHUMP00000020813 antibody|OTTHUMP00000046571 antibody|P450 ALC antibody|p450 antibody|P450 J antibody|P450-J antibody|P450C2E antibody|P450J antibody|P450RLM6 antibody|Xenobiotic monooxygenase antibody

Application Details

Application	Concentration*	Species	Validated Using**
Western blot	0.1-0.5μg/ml	Mouse, Rat	AssaySolutio's ECL kit
Immunohistochemistry(Paraffin-embedded Section)	0.5-1μg/ml	Mouse, Rat	AssaySolutio's IHC/ICC Detection kit

AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P). *Blocking peptide can be purchased at $65. Contact us for more information