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Overview
Long Name | Antibody Type | Antibody Isotype | Host | Species Reactivity | Validated Applications | Purification |
| cystatin C | Polyclonal | IgG | Rabbit | Human | IHC-P, ICC, WB | Immunogen affinity purified. |
Immunogen | ||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of human Cystatin C(45-63aa EEEGVRRALDFAVGEYNKA). | ||||||
Properties
Form | Lyophilized |
Size | 100 µg/vial |
Contents | Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration | Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage | At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene | CST3 |
Protein | Cystatin-C |
Uniprot ID | P01034 |
Function | As an inhibitor of cysteine proteinases, this protein is thought to serve an important physiological role as a local regulator of this enzyme activity. |
Tissue Specificity | Expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Expressed in various body fluids, such as the cerebrospinal fluid and plasma. Expressed in highest levels in the epididymis, vas deferens, brain, thymus, and ovary and the lowest in the submandibular gland. |
Sub-cellular localization | Secreted. |
Sequence Similarities | |
Aliases | AD 8 antibody|AD8 antibody|Amyloid angiopathy and cerebral hemorrhage antibody|ARMD11 antibody|bA218C14.4(cystatin C) antibody|bA218C14.4 antibody|Cst 3 antibody|Cst3 antibody|CST3 protein antibody|Cystatin 3 antibody|Cystatin-3 antibody|Cystatin-C antibody|Cystatin3 antibody|CystatinC antibody|CYTC_HUMAN antibody|Gamma trace antibody|Gamma-trace antibody|HCCAA antibody|MGC117328 antibody|Neuroendocrine basic polypeptide antibody|Post gamma globulin antibody|Post-gamma-globulin antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio's ECL kit |
| Immunohistochemistry(Paraffin-embedded Section) | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
| Immunocytochemistry | 0.5-1μg/ml | Human | AssaySolutio's IHC/ICC Detection kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot, and Rabbit Peroxidase IHC/ICC Detection Kit (AKIT002B) for IHC(P) and ICC. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-Cystatin C antibody, ASA-B0558, Western blotting
Lane 1: Recombinant Human Cystatin C Protein 10ng
Lane 2: Recombinant Human Cystatin C Protein 5ng
Lane 3: Recombinant Human Cystatin C Protein 2.5ng
Lane 1: Recombinant Human Cystatin C Protein 10ng
Lane 2: Recombinant Human Cystatin C Protein 5ng
Lane 3: Recombinant Human Cystatin C Protein 2.5ng
Anti-Cystatin C antibody, ASA-B0558, IHC(P)
IHC(P): Human Mammary Cancer Tissue
IHC(P): Human Mammary Cancer Tissue
Anti-Cystatin C antibody, ASA-B0558, ICC
ICC: MCF-7 Cell
ICC: MCF-7 Cell