Data sheet
| Assay Range | 156.25 - 10000 pg/ml |
More info
Sensitivity
150 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human VNN2 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human VNN2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human VNN2 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
VNN2 (Vascular non-inflammatory molecule 2), is a cell membrane protein which belongs to the CN hydrolase family and Vanin subfamily. It is an amidohydrolase that hydrolyzes specifically one of the carboamide linkages in D-pantetheine thus recycling pantothenic acid (vitamin B5) and releasing cysteamine. It is involved in the thymus homing of bone marrow cells. VNN2 plays a role in transendothelial migration of neutrophils and may regulate beta-2 integrin-mediated cell adhesion, migration and motility of neutrophil.
