More info
Assay Range | 31.2-2,000 pg/mL |
Sensitivity | 1.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human VEGF standard: 2 vials
2. One 96-well plate coated with Human VEGF Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Vascular endothelial growth factor (VEGF or VEGF-A), also known as vascular permeability factor (VPF), is a member of the PDGF family characterized by the presence of eight conserved cysteine residues in a cysteine knot structure and the formation of antiparallel disulfide-linked dimers. Six alternately spliced VEGF isoforms containing 121, 145, 165, 183, 189, and 206 amino acids (aa), respectively, have been identified in humans. VEGF165 appears to be the most abundant and potent isoform and shares 88% aa sequence identity with mouse and rat VEGF within corresponding regions. VEGF isoforms are differentially expressed during development and in the adult.
VEGF dimers bind to two related receptor tyrosine kinases, VEGF R1 (Flt-1) and VEGF R2 (Flk-1/KDR) to activate a series of cellular processes. During embryogenesis, VEGF regulates proliferation, migration, and survival of endothelial cells, and modulate blood vessel density and size. In adulthood, VEGF functions primarily in wound healing and the female reproductive cycle.