Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
15 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant humanTF standard: 5 ng/vial - 2.
2. One 96-well plate precoated with anti-human TF Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human TF Ab: 1 vial, dilution 1:400.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Transferrin is a monomeric serum glycoprotein that binds up to two Fe3+ atoms for delivery to vertebrate cells through receptor-mediated endocytosis. Transferrin is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin-receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Serum transferrin may also have a further role in stimulating cell proliferation.
