Data sheet
| Assay Range | 62.5 - 4000 pg/ml |
More info
Sensitivity
60 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human PIGR standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human PIGR Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human PIGR Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
PIGR (Polymeric Immunoglobulin Receptor) is a 100-kDa type I transmembrane glycoprotein. It is expressed on secretory epithelial cells of exocrine tissues. It functions as a carrier that transports IgA and IgM across epithelium. This receptor binds polymeric IgA and IgM at the basolateral surface of epithelial cells. The complex is then transported across the cell to be secreted at the apical surface. During this process a cleavage occurs that separates the extracellular (known as the secretory component) from the transmembrane segment.
