Data sheet
| Assay Range | 78.125 - 5000 pg/ml |
More info
Sensitivity
70 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human NAALADL1 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human NAALADL1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human NAALADL1 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Hydrolysis of the neuropeptide N-acetyl-L-aspartyl-L-glutamate (NAAG) by N-acetylated α-linked acidic dipeptidase (NAALADase) to release glutamate may be important in a number of neurodegenerative disorders in which excitotoxic mechanisms are implicated. NAALADL1 (N-acetylated α-linked acidic dipeptidase-like 1), also known as I100 or NAALADASEL, is a 740 amino acid single-pass type II membrane protein. Belonging to the peptidase M28 family and the M28B subfamily, NAALADL1 is mainly expressed in the distal small intestine, spleen and testis. It is also weakly expression in the brain, locating mainly to the brain stem, amygdala, thalamus and ventral striatum. NAALADL1 is considered a novel ileal brush border membrane protein.
