Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human MSR1 standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-human MSR1 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human MSR1 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD204, also called scavenger receptor A, is a phagocytic pattern-recognition receptors (PRRs) expressed primarily on macrophages and dendritic cells (DCs).This receptor includes three different types (1, 2, 3) generated by alternative splicing on mRNA. These receptors or isoforms are trimeric integral membrane glycoproteins and have been implicated in many macrophage-associated physiological and pathological processes including atherosclerosis, Alzheimer's disease, and host defense.
