Data sheet
| Assay Range | 78 - 5000pg/ml |
More info
Assay Format
Sandwich ELISA.
Storage
Store at 4 °C.
Expiration
12 months at 4 °C.
Range
78 pg/mL - 5000 pg/mL.
Sensitivity
50 pg/mL.
Specificity
No detectable cross-reactivity with any other cytokine.
Application
cell culture supernatants, serum and plasma.
Kit Components
1. Lyophilized recombinant Human Mesothelin standard: 2.5 ng/tube-2.
2. One 96-well plate precoated with anti-Human Mesothelin Ab.
3. Sample diluent buffer: 12 mL -2
4. Biotinylated anti-Human Mesothelin antibody: 200µL, dilution 1:60.
5. Antibody diluent buffer: 12ml.
6. Streptavidin-HRP: 300 µL, dilution 1:40.
7. Streptavidin-HRP diluent buffer: 12ml.
8. Chromogen Solution A (H2O2): 6ml.
9. Chromogen Solution B (TMB): 6ml.
10. Stop Solution: 6 ml.
Product Citation:
1. Reduced Shedding of Surface Mesothelin Improves Efficacy of Mesothelin-Targeting Recombinant Immunotoxins
Prince Awuah, Tapan K. Bera, Messan Folivi, Oleg Chertov and Ira Pastan
Mol Cancer Ther July 1 2016 (15) (7) 1648-1655; DOI: 10.1158/1535-7163.MCT-15-0863
Prince Awuah, Tapan K. Bera, Messan Folivi, Oleg Chertov and Ira Pastan
Mol Cancer Ther July 1 2016 (15) (7) 1648-1655; DOI: 10.1158/1535-7163.MCT-15-0863