More info
Assay Range | 31.2-2,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human IL-16 standard: 2 vials
2. One 96-well plate coated with Human IL-16 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Interleukin 16 (IL-16), formerly known as lymphocyte chemoattractant factor (LCF), is a proinflammatory cytokine encoded by IL-16 gene. It is chemotactic for CD4+ T lymphocytes, monocytes and eosinophils. IL-16 mRNA is constitutively expressed in both CD4+ and CD8+ cells. CD4 functions as a receptor for IL-16 and is required for IL-16 mediated signal transduction. IL-16 can initiate an increase in cytoplasmic calcium and inositol trisphosphate, activation of p56lck, and translocation of protein kinase C from the cytosol to the cell membrane by interacting with CD4. IL-16 expression has been linked to inflammation processes in asthma, rheumatoid arthritis, systemic lupus erythematosus, colitis, atopic dermatitis, and multiple sclerosis.