Data sheet
| Assay Range | 78.125 - 5000 pg/ml |
More info
Sensitivity
50 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human C2 standard: 10 ng/vial - 2.
2. One 96-well plate precoated with anti-human C2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human C2 Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
C2 is a glycoprotein synthesized in liver hepatocytes and several other cell types in extrahepatic tissues. Activated C1 cleaves C2 into C2a and C2b. C2a leads to activation of C3. Deficiency of C2 has been reported to associate with certain autoimmune diseases. Defects in C2 are the cause of complement component 2 deficiency (C2D). C2D is reported to increase susceptibility to infection, and is associated with certain autoimmune diseases.
