Data sheet
| Assay Range | 31.2 - 2000 pg/ml |
More info
Sensitivity
15 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD32b standard: 2 ng/vial - 2.
2. One 96-well plate precoated with anti-human CD32b Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD32b Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD32b, also known as FCGR2B, is a "low affinity" receptor for Immunoglobulin G. The inhibitory CD32b is expressed on B cells and myeloid dendritic cells. CD32b is the inhibitory FcR and the inhibitory capacity of CD32b is largely dependent on expression of an intracellular ITIM. Ligation of CD32b on B cells downregulates antibody production and may promote apoptosis. Co-ligation of CD32b on dendritic cells inhibits maturation and blocks cell activation. CD32b may also be a target for monoclonal antibody therapy for malignancies. Deficiency in CD32b leads to chronic B cell activation and autoimmunity. Polymorphisms in CD32b are associated with an increased prevalence of SLE.
