Data sheet
| Assay Range | 46.875 - 3000 pg/ml |
| Sensitivity | 20 pg/ml |
| Size | 96T |
| Storage | Store at 2 - 8⁰C. Keep Standard and detection antibody at -20⁰C. |
| Expiration | Six months at 2 - 8 ⁰C. |
| Application | For quantitative determination of Human ACRV1. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Sample type | Culture Supernates, Serum, plasma, body fluids, tissue lysates. |
More info
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant ACRV1 standard: 3ng/ vial - 2.
2. One 96-well plate precoated with anti-ACRV1 antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-ACRV1 antibody: 30µL, dilution 1:500.
5. Detection Antibody diluention buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 12 ml.
8. TMB color developing agent B: 12 ml.
9. TMB stop solution: 6ml.
Background
Acrosomal protein SP-10, also known as Acrosomal vesicle protein 1 and ACRV1, is a testis-specific, differentiation antigen, that arises within the acrosomal vesicle during spermatogenesis, and is associated with the acrosomal membranes and matrix of mature sperm. ACRV1 / SP-10 is a testis-specific acrosomal protein that has been detected in several species including humans. Regulation of cell type-specific gene transcription is central to cellular differentiation and development.
