More info
Product name
Human OPG ELISA Kit
Catalog No.
FM-E100111
Size
96T
Range
93.8pg/mL-6000pg/mL
Sensitivity
Less Than 5 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
For quantitative detection of human OPG in serum,
body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant human OPG standard: 10ng/tube-2.
2. One 96-well plate precoated with anti- human OPG antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- human OPG antibody: 130µL, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
Osteoprotegerin (OPG) is identical to osteoclastogenesis inhibitory factor (OCIF), a soluble member of the tumor-necrosis factor receptor family that inhibits osteoclastogenesis. OPG is considered to play an important role in the regulation of bone resorption by modifying osteoclast differentiation. Osteoprotegerin is an independent risk factor for the progression of atherosclerosis and onset of cardiovascular disease. It can act as a soluble factor in the regulation of bone mass and imply a utility for OPG in the treatment of osteoporosis associated with increased osteoclast activity. OPG system may play a critical role in the development of osteolytic bone disease in multiple myeloma and that targeting this system may have therapeutic potential. OPG also plays a role in the vascular system. The standard product used in this kit is recombinant OPG, a 55KDa glycoprotein.
Human OPG ELISA Kit-1X96 Well Plate Image