Human MMP-9 ELISA Kit

Kit Components

 1. Recombinant Human MMP-9 standard: 2 vials

 2. One 96-well plate precoated with anti- Human MMP-9 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

Matrix metalloproteinase-9 (MMP-9), also known as gelatinase B, is a member of the matrix metalloproteinases (MMPs) family belonging to the zinc and calcium dependent endopeptidase superfamily. Matrix Metalloproteinases (MMPs), also called Matrixins, plays an important role in the degradation of the extracellular matrix which is involved in a series of physiological processes related to embryonic development, morphogenesis, and tissue remodeling. MMPs have also been implicated in the pathogenesis of many diseases such as arthritis, cancer and cardiovascular disease. Human MMP-9 is secreted as a proenzyme (pro-MMP-9) composed of three fibronectin type II domains, a hemopexin-like domain and a proline-rich type V collagen-homologous domain. Pro-MMP-9 can be activated by MMP-3 or certain bacterial proteases. MMP-9 is inhibited by α2-macroglobulin or TIMP-1. Pro-MMP-9 is secreted by monocytes, macrophages, neutrophils, keratinocytes, fibroblasts, osteoclasts, chondrocytes, skeletal muscle satellite cells, endothelial cells, and various tumor cells. Expression of pro-MMP-9 is induced by TGF-β1, IL-1β, TGF-α, PDGF-AB, TNF-α, and IL-1α. MMP-9 can degrade denatured collagen type I (gelatin), native collagens type IV, V, VII, X and XI, fibrinogen, vitronectin, IL-1β, and entactin.