More info
Product name
Human BMP-5 ELISA Kit
Catalog No.
FM-E100008
Size
96T
Range
62.5pg/mL-4000pg/mL
Sensitivity
Less Than 10pg/mL
Specificity
Detectable cross-reactivity with BMP Less Than 0.2%.
Storage
Store at 4 for frequent use, at -20 for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
Four months at 4 and eight months at -20.
Application
serum, plasma, body fluids, tissue lysates or cell culture supernates.
Kit Components
1. Lyophilized recombinant human BMP-5 standard: 10ng/tube-2.
2. One 96-well plate precoated with anti- human BMP-5 antibody.
3. Sample diluent buffer: 30 ml
4. Biotinylated anti- human BMP-5 antibody: 130µL, dilution 1:100.
5. Antibody diluent buffer: 12ml.
6. Avidin-Biotin-Peroxidase Complex (ABC): 130µL, dilution 1:100.
7. ABC diluent buffer: 12ml.
8. TMB color developing agent: 10ml.
9. TMB stop solution: 10ml.
Background
Bone morphogenetic protein 5 is a protein that in humans is encoded by the BMP5 gene.1,2,3 The protein encoded by this gene is member of the TGFβ superfamily. Bone morphogenetic proteins are known for their ability to induce bone and cartilage development. BMP5 may play a role in certain cancers. Like other BMP's BMP5 is inhibited by chordin and noggin. It is expressed in the trabecular meshwork and optic nerve head and may have a role in the development and normal function. It is also expressed in the lung and liver. BMP-5 is mapped on human chromosome 6.4 The standard used in this kit is recombinant BMP-5 with the monomer molecular mass of 18KDa.
Human BMP-5 ELISA Kit-1X96 Well Plate Image