More info
Principle
Sandwich ELISA
Detection Range
0 - 30 µg/ml
Size
96T
Specificity 95%
Assay time
~ 90 min
Shelf Life
12~14 Months from the Manufacturing Date
Storage
Store at 2 - 8ºC
Sample
Serum, Plasma
Detection Method
Chromogenic
Kit Components
1. standard Set: 6 vials
2. One 96-well plate precoated with antibody
3. Assa diluent buffer: 1 vial
4. PAPP-A-HRP conjugate: 1 vial
5. 25 x wash buffer:1bottle
6. TMB substrate: 1 vial
7. TMB stop solution: 12 ml.
Background
Pappalysin-1 also known as pregnancy-associated plasma protein A (PAPP-A) is a protein that in humans is encoded by the PAPPA gene. The hetreotetramer consists of two PAPP-A subunits and two proMBP subunits. Pappalysin-1 is used in screening tests for Down syndrome. It involves in local proliferative processes such as wound healing and bone remodeling. Low plasma level of this protein has been suggested as a biochemical marker for pregnancies with aneuploidfetuses (fetuses with an abnormal number of chromosomes). Low levels may alternatively predict issues with the placenta, resulting in adverse complications such as intrauterine growth restriction, preeclampsia, placental abruption, premature birth, or fetal death.
