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Human Osteoprotegerin OPG ELISA Kit
BG-HUM11612
Human Osteoprotegerin (OPG) ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction
Data sheet
| Assay Range | 62.5 -- 4000 pg/mL |
| Size | 96T |
| Storage | 2 - 8 ℃ |
| Background | Osteoprotegerin (OPG), also known as osteoclastogenesis inhibitory factor (OCIF) or tumour necrosis factor receptor superfamily member 11B (TNFRSF11B), is a cytokine receptor of the tumour necrosis factor (TNF) receptor superfamily encoded by the TNFRSF11B gene. OPG was first discovered as a novel secreted TNFR related protein that played a role in the regulation of bone density and later for its role as a decoy receptor for Receptor Activator of Nuclear Factor kappa-B ligand (RANKL). OPG also binds to TNF-related apoptosis-inducing ligand (TRAIL) and inhibits TRAIL induced apoptosis of specific cells, including tumour cells. Other OPG ligands include syndecan-1, glycosaminoglycans, von Willebrand Factor, and Factor VIII-von Willebrand Factor complex.OPG has been identified as having a role in tumour growth and metastasis, heart disease, immune system development and signalling, mental health,diabetes, and the prevention of pre-eclampsia and osteoporosis during pregnancy. |
| Assay principle | The Human Osteoprotegerin/TNFRSF11B ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Human Osteoprotegerin/TNFRSF11B specific antibody has been pre-coated onto 96-well plate. Human Osteoprotegerin/TNFRSF11B present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Human Osteoprotegerin/TNFRSF11B detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Human Osteoprotegerin/TNFRSF11B captured on plate. |
| INTENDED USE | The kit is used to quantify the Human Osteoprotegerin/TNFRSF11B in serum/ plasma, cell culture supernatant and other suitable sample solution. |
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Human Osteoprotegerin ELISA Kit
Citations:
Low bone mineral density among young healthy adult Saudi women
Prevalence and associated factors in the age group of 20 to 36 years
Zeidan A. Zeidan, Intisar E. Sultan, Shaista S. Guraya
Saudi Medical Journal, 2016 Nov; 37(11): 1225–1233.