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Human Hepcidin ELISA Kit
BG-HUM11160
Human Hepcidin (Hepc) ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction Competition
Data sheet
| Size | 96T |
| Assay principle | The Human Hepcidin ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Human Hepcidin specific antibody has been pre-coated onto 96-well plate. Human Hepcidin present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Human Hepcidin detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Human Hepcidin captured on plate. |
| Amino acid sequence | ICIFCCGCCHRSKCGMCCKT |
| Alternate Names | HAMP; HEPC; hepcidin antimicrobial peptide; Hepcidin; HEPCPutative liver tumor regressor; HFE2B; HFE2Bhepcidin; LEAP1; LEAP-1; LEAP1PLTR; Liver-expressed antimicrobial peptide 1; PLTR |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric ELISA |
| Assay Time | 4.5 hours |
| Sample Type | Plasma, Serum, Urine |
| Assay Range | 12.5 – 800 pg/ml |
| Sensitivity | 1.25 pg/ml |
| Specificity | recognize both native and well folded recombinant human hepcidin |
| Recovery | 94% to 113% |
| Precision | Intra-Assay, 5.6%; Inter-Assay, 9.9% |
| Sample Volume | 100 ul |
| Dilution Factors | 5 to 60 folds for plasma and serum |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Gene ID | 57817 |