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Human HMGB1 ELISA Kit
BG-HUM11133
Human High Mobility Group Box1 Protein ELISA Kit
Data sheet
| Assay principle | The Human HMGB1 ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse anti-Human HMGB1 specific antibody has been pre-coated onto 96-well plate. The test samples and the biotinylated goat anti-Human HMGB1 specific detection antibody are added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP is added and unbound conjugates are washed away with Wash Buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic Stop Solution. The density of yellow is proportional to the amount of Human HMGB1 captured on plate. |
| Alternate Names | Amphoterin; high mobility group box 1; High mobility group protein 1; high mobility group protein B1; high-mobility group (nonhistone chromosomal) protein 1; high-mobility group box 1; HMG1; HMG-1; HMG1DKFZp686A04236; HMG3; HMGB1; SBP-1; Sulfoglucuronyl carbohydrate binding protein |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric ELISA |
| Assay Time | 4.5 hours |
| Sample Type | Plasma, Serum, Cell culture medium, Cell or tissue lysate. |
| Assay Range | 1.56 - 100 ng/ml |
| Sensitivity | 0.3 ng/ml |
| Specificity | No significant cross-reactivity or interference between Human HMGB1 and analogues was observed. |
| Recovery | 91 - 107% |
| Precision | Intra-Assay 4.8%; Inter-Assay 9.7%. |
| Sample Volume | 100 ul |
| Dilution Factors | Undiluted for plasma and serum |
| Storage | Storage of components varies. See protocol for specific instructions. |
| Gene ID | 3146 |
| UniProt ID | P09429 |
More info
| Precision | ||||||
| Intra-Assay Precision | Inter-Assay Precision | |||||
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 20 | 20 | 20 | 20 | 20 | 20 |
| CV (%) | 4.6 | 4.8 | 5.1 | 8.9 | 9.7 | 10.5 |
| Average CV (%) | 4.8 | 9.7 | ||||
| Recovery | ||||||
| Standard Added Value | 12 - 100 ng/ml | |||||
| Recovery % | 91 - 107% | |||||
| Average Recovery % | 98% | |||||
| Linearity | ||||||
| Average Percentage of Expected Value (%) | ||||||
| Sample Dilution | Plasma | Serum | ||||
| 2 x | 105% | 96% | ||||
| 4 x | 101% | 99% | ||||
| 8 x | 98% | 97% | ||||
PRODUCT CITATIONS
1. Systemic immune response associated with radiation therapy in B‑cell non‑Hodgkin's lymphoma of Waldeyer's ring, Niu XJ et al, Oncology Reports, December 2018, Volume 40 Issue 6
2. HMGB1 and thrombin mediate the blood-brain barrier dysfunction acting as biomarkers of neuroinflammation and progression to neurodegeneration in Alzheimer’s disease, Barry W. Festoff, Ravi K. Sajja, Patrick van Dreden and Luca Cucullo, Journal of Neuroinflammation 2016 13:194 https://doi.org/10.1186/s12974-016-0670-z
3. S100B promotes glioma growth through chemoattraction of myeloid-derived macrophages. Wang H, Zhang L, Zhang IY, Chen X, Da Fonseca A, Wu S, Ren H, Badie S, Sadeghi S, Ouyang M, Warden CD, Badie B. Clin Cancer Res. 2013 Jul 15;19(14):3764-75