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Human CD19 ELISA Kit

NS-E10186

Human Cluster Of Differentiation 19 ELISA KitFor research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction

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$599.00

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Assay Format
Sandwich ELISA. 
 
Storage
Store at 4 °C.
 
Expiration
6 months at 4 °C.
 
Range
156 pg/mL - 10000 pg/mL.
 
Sensitivity
150 pg/mL.
 
Specificity
No detectable cross-reactivity with any other cytokine.
 
Application
cell culture supernatants, serum and plasma.
 
 
 
 
Kit Components
 
 
 
1. Lyophilized recombinant Human CD19 standard: 10 ng/tube-2.
 
 
2. One 96-well plate precoated with anti-Human CD19 antibody.
 
 
3. Sample diluent buffer: 10 mL - 1
 
 
4.  Anti-Human CD17 detection antibody-1, dilution 1:2000.
 
 
5. Antibody diluent buffer: 10ml - 1.
 
 
6. Chromogenic Solution A: 6ml - 1.
 
 
7. Chromogenic Solution B: 6ml - 1.
 
 
8. Stop solution: 10ml - 1.
 
Background

 

B-lymphocyte antigen CD19, also known as CD19, is a single-pass type I membrane protein which was assembled with the antigen receptor of B-lymphocytes in order to decrease the threshold for antigen receptor-dependent stimulation. CD19 is an important differentiation antigen of the B cell lineage and positively regulates antigen receptor signal transduction in mature B cells. CD19 forms a complex with CD21, CD81 and CD225 in the membrane of mature B-cells. It interacts with VAV, GRB2 and SOS when phosphorylated on Tyr-348 and/or Tyr-378.  CD19 also interacts with PLCG2 when phosphorylated on Tyr-409. Defects in CD19 are a cause of hypogammaglobulinemia and results in common variable immunodeficiency.

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