More info
Assay Range | 156-10,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Angiopoietin-1 standard: 2 vials
2. One 96-well plate precoated with anti-Human Angiopoietin-1 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Angiopoietin-1 (Ang-1) is a member of the vascular growth factor family that also includes Ang-2 and Ang-4 in humans. Ang-1 is a secreted glycoprotein characteristic of an N-terminal coiled-coil domain and a C-terminal fibrinogen-like domain. Ang-1 exhibits approximately 60% aa sequence identity with Ang-2 and both of them act as a ligand for the receptor tyrosine kinase Tie-2. Integrins may also mediate Ang-1 and Ang-2 activities. Ang-1 is widely expressed in the mouse embryo and in adult human tissues, primarily by endothelial support cells, megakaryocytes and platelets. Ang-1 plays a key role in blood vessel development, remodeling, and maturation. In addition, Ang-1 may help maintain hematopoietic stem cells in a quiescent state within the bone marrow. It also enhances neutrophil and eosinophil adhesion and migration, and regulates blood vessel permeability. In the nervous system, Ang-1 may support neuronal growth, survival, and dendritic organization.