More info
Assay Range | 15.6-1,000 pg/mL |
Sensitivity | 10.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human Tissue factor standard: 2 vials
2. One 96-well plate coated with anti-Human Tissue factor Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
Background
Tissue Factor, also known as Coagulation Factor III, Thromboplastin, and CD142, is a transmembrane protein composed of an extracellular domain, a transmembrane domain, and a cytoplasmic tail. In the vessel wall, Tissue Factor is constitutively expressed in subendothelial cells, such as vascular smooth muscle cells. Tissue Factor is induced in endothelial cells and monocytes in response to stimuli of cytokines and growth factors. Tissue Factor is essential for the coagulation cascade and is the primary initiator of the extrinsic coagulation pathway. It functions as the high-affinity receptor for the coagulation factor VII. Once Coagulation Factor VII is complexed with Tissue Factor, it is converted to the active form (FVIIa). The Tissue Factor- FVIIa complex then proteolytically activates downstream coagulation factors, including Coagulation Factor IX and Coagulation Factor X, ultimately leading to the conversion of prothrombin to thrombin and fibrin clot formation. Tissue Factor also plays an important role in pathological conditions related to hemostasis, such as thrombotic diseases and disseminated intravascular coagulation. In addition, Tissue Factor has been associated with non-hemostatic processes, such as inflammation, angiogenesis, and tumor growth and metastasis. It is reported that that mice lacking Tissue Factor die at the embryonic stage due to abnormal development of vasculature. Furthermore, It has been shown that Tissue Factor -FVIIa complex contribute to apoptosis resistance and increased survival of cancer cell lines.