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Lipoteichoic acids, LTA ELISA Kit
Lipoteichoic acids,LTA ELISA Kit For research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction
Data sheet
| Background | Lipoteichoic acid (LTA) is a major constituent of the cell wall of gram-positive bacteria. These organisms have an inner (or cytoplasmic) membrane and, external to it, a thick (up to 80 nanometer) peptidoglycan layer. The structure of LTA varies between the different species of Gram-positive bacteria and may contain long chains of ribitol or glycerol phosphate. LTA is anchored to the cell membrane via a diacylglycerol. It acts as regulator of autolytic wall enzymes (muramidases). It has antigenic properties being able to stimulate specific immune response. LTA may bind to target cells non-specifically through membrane phospholipids, or specifically to CD14 and to Toll-like receptors. Binding to TLR-2 has shown to induce NF-κB expression(a central transcription factor), elevating expression of both pro- and anti-apoptotic genes. Its activation also induces mitogen-activated protein kinases (MAPK) activation along with phosphoinositide 3-kinase activation. LTA's molecular structure has been found to have the strongest hydrophobic bonds of an entire bacteria. Said et al. showed that LTA causes an IL-10-dependent inhibition of CD4 T-cell expansion and function by up-regulating PD-1 levels on monocytes which leads to IL-10 production by monocytes after binding of PD-1 by PD-L. |
| Assay Type | Sandwich ELISA |
More info
|
Assay Range |
156-10,000 pg/mL |
|
Sensitivity |
75 pg/mL |
|
Specificity |
No cross-reaction with other related substances detected |
|
Size |
96T |
|
Storage |
Store at 2 - 8 ºC. |
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Assay Principle |
Sandwich ELISA |
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Sample Volume |
100 µL final volume, dilution factor varies on samples |
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Detection Method |
Chromogenic |
Structure:
Kit Components
1. Standard: 0.5 mL x 6 vials
2. One 96-well plate coated with capture Ab
3. Sample diluent buffer: 6 ml
4. HRP conjugate reagent: 10 ml
5. Chromogen solution A: 6 ml
6. Chromogen solution B: 6 ml
7. Stop solution: 6 mL x1
8. Washing solution (20x): 25 mL x1
9. Adhesive plate sealers: 2
10. Package insert: 1