More info
Assay Range | 0.125-2.0 µg/ml |
Sensitivity | 0.1 µg/ml |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 50 µL final volume, dilution factor varies on samples |
Sample Type | serum, plasma, body fluids, tissue lysate or cell culture supernatant |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human SAA-1 in standard: 0.5 ml
2. One 96-well plate coated with Human SAA-1 Ab
3. Sample diluent buffer (10x): 30 mL - 1
4. Detection antibody: 140 µL, dilution 1:50
5. Streptavidin-HRP: 80 µL, dilution 1:100
6. Streptavidin-HRP diluent buffer: 12 mL x1
7. TMB developing agent: 8 mL x1
8. Stop solution: 12 mL x1
9. Washing solution (20x): 30 mL x 2
Background
Human Serum Amyloid A-1 (SAA-1) is a major apolipoprotein of high-density lipoprotein in plasma and a sensitive marker of acute inflammation. Four SAA genes have been identified and three encode functional proteins in human. SAA is a 12.5-kDa protein containing 122 amino acids with polymorphic forms. It is synthesized by the liver and adipose tissue, and also produced extrahepatically by many cancers. In response to inflammatory stimuli, acute-phase SAA-1 and SAA-2 are secreted and increased. SAA-3 is a pseudogene that does not express protein. SAA-4 is expressed constitutively in the liver. SAA is associated with obesity, amyloidosis, type 2 diabetes, atherosclerosis, metabolic syndrome, rheumatoid arthritis, and renal and lung cancers.