More info
Assay Range | 62.5--4000 pg/mL |
Sensitivity | 4.0 pg/mL |
Size | 96T |
Storage | Store at 2 - 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Recombinant Human BMP-4 standard: 2 vials
2. One 96-well plate precoated with anti-Human BMP-4 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1.
10. Washing solution (20x): 25 mL x1.
Background
Bone morphogenetic protein-4 (BMP-4), also known as BMP-2b, is a member of the transforming growth factor beta (TGF-β) superfamily. BMPs were originally identified as protein regulators of cartilage and bone formation. Extensive studies have shown that BMP-4 plays an important role in the onset of endochondral bone formation, dorsal/ventral patterning. The BMP-4 precursor is a 408 amino acid (aa) protein containing a 19 aa signal sequence, a 273 aa pro-region, and a 116 aa mature segment. Both the pro-region and mature segment contain two potential N-linked glycosylation sites. The mature region consists of seven highly conserved cysteine aa residues featured in TGF-β superfamily members. The mature region of human BMP-4 shares 98% aa sequence identity with mouse and rat BMP-4. BMP-4 binds to ALK-6/BMP RIB and BMP RII to activate a series of biological processes in which smad proteins play a critical role.