Data sheet
| Assay time | 4.5 Hours |
| Validity | 6 months |
| Assay Range | 0.62 – 40 ng/mL |
| Assay principle | The Human Osteocalcin ELISA Kit is based on standard sandwich enzyme-linked immunosorbent assay technology. Anti-Human Osteocalcin specific antibody has been pre-coated onto 96-well plate. Human Osteocalcin present in the standards/ samples bind to the capture antibody. Subsequently, biotinylated anti-Human Osteocalcin detection antibody is added to form an Ab-Ag-Ab sandwich. After a washing step, streptavidin-HRP is added and the unbound conjugate is removed with wash buffer. Next, addition of HRP substrate, TMB, results in the production of a blue colored product that changes to yellow after the addition of acidic Stop Solution. The density of yellow color is directly proportional to the amount of Human Osteocalcin captured on plate. |
More info
Product Citation:
1. Low bone mineral density among young healthy adult Saudi women
Prevalence and associated factors in the age group of 20 to 36 years, Zeidan A. Zeidan et al, Saudi Med J. 2016 Nov; 37(11): 1225–1233.