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Assay principle
This assay employs the enzyme-linked immunosorbent assay (ELISA) technology to detect anti- Hepatitis C virus antigen (HCV) IgG in the samples. Hepatitis C virus antigen peptides were precoated in microplate wells. The anti-HCV IgG in the samples, if presents, will bind to the Hepatitis C virus antigen peptides immobilized on the wells and interacts with anti-Human IgG antibody-HRP conjugate to form an immunocomplex. Following incubation and washing procedures to remove unbound substances, this reaction is visualized by the addition of the chromogen tetramethylbenzidine (TMB). After stopping the reaction with sulfuric acid, the blue color turns yellow. What can be measured at this point is the amount of color intensity proportional to the amount of antibody captured in the wells, and to the sample.
Kit Components
1 | Microplate precoated with HCV antigens | 1x96 well |
2 | Negative Control | 0.2 ml X 1 vial |
3 | Positive Control | 0.2 ml X 1 vial |
4 | Diluent buffer | 13 mL x1 |
5 | Wash Solution (20x) | 50 mL x1 |
6 | Anti-Human IgG-HRP Conjugate | 13 ml x1 |
7 | Chromogenic Solution A | 8 ml |
8 | Chromogenic Solution B (TMB) | 8 ml |
9 | Stop Solution | 8 ml |