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Human S100 calcium binding protein A8/calgranulin A, S100A8 ELISA Kit
human S100 calcium binding protein A8/calgranulin A,S100A8 ELISA Kit For research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA
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Assay Range | 31.25-2,000 pg/mL |
Assay Time | 4 hours 40 minutes |
Specificity | No cross-reaction with other related substances detected |
Size | 96T |
Storage | Store at 2 - 8 ºC. Store reconstituted standard and detection Ab at -20 ºC |
Assay Principle | Sandwich ELISA |
Sample Volume | 100 µL final volume, dilution factor varies on samples |
Detection Method | Chromogenic |
Kit Components
1. Human S100A8 standard: 2 vials
2. One 96-well plate coated with anti-Human S100A8 Ab
3. Sample diluent buffer: 12 mL - 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1