Human tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit

Kit Components

 1. Recombinant Human TIMP-2 standard: 2 vials

 2. One 96-well plate precoated with anti- Human TIMP-2 Ab

 3. Sample diluent buffer: 12 mL - 1

 4. Detection antibody: 130 µL, dilution 1:100

 5. Streptavidin-HRP: 130 µL, dilution 1:100

 6. Antibody diluent buffer: 12 mL x1   

 7. Streptavidin-HRP diluent buffer: 12 mL x1

 8. TMB developing agent: 10 mL x1

9. Stop solution: 10 mL x1.

10. Washing solution (20x): 25 mL x1.

Background

The tissue inhibitor of metalloproteinase 2 (TIMP-2), also known as CSC-21K, is a member of the tissue inhibitor of metalloproteinase (TIMP) family in which four proteins TIMP1, TIMP2, TIMP3 and TIMP4 have been identified so far. TIMPs are natural inhibitors of the matrix metalloproteinases (MMPs) which are involved in degradation of the extracellular matrix and exhibit diverse functions in development, tissue remodeling, wound healing and tumor metastasis. TIMPs block access of substrates to the MMP catalytic site by forming 1:1 non-covalent complexes with MMPs. Basically, TIMPs are highly specific for MMPs but may not be able to digest some particular MMP such as MMP14. TIMP-2 is required for activation of pro-MMP-2 by active MMP-14. In addition to its inhibitory activity on MMPs, it suppresses EGF-mediated mitogenic signaling. TIMP-2 expression is inducible in certain pathological conditions. For instance, elevated levels of TIMP-2 in sera of patients with systemic sclerosis have been observed and are associated with the extent of skin sclerosis. Decreased levels of TIMP-2 in ovarian carcinoma cells and in tracheal aspirate fluid from preterm infants with respiratory distress have also been detected. Furthermore, TIMP-2 and TIMP-1 expression is significantly higher in grade I human brain tumors than in grade II and III, strongly suggesting that their expression may be valuable markers for tumor malignancy.