Data sheet
| Storage | 2 - 8 ℃ |
| Background | WAP four-disulfide core domain protein 2 - also known as Human Epididymis Protein 4 (HE4) - is a protein that in humans is encoded by the WFDC2 gene. HE4 is a tumor marker of ovarian cancer, with 80% sensitivity at a cut-off of 150 pmol/L. This gene encodes a protein that is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. This gene is expressed in pulmonary epithelial cells, and was also found to be expressed in some ovarian cancers. The encoded protein is a small secretory protein, which may be involved in sperm maturation. |
| Alternate Names | HE4; HE4MGC57529; Major epididymis-specific protein E4; Putative protease inhibitor WAP5; WAP domain containing protein HE4-V4; WAP four-disulfide core domain 2; WAP four-disulfide core domain protein 2; WAP5; WAP5epididymal protein 4; WFDC2, EDDM4 |
| Assay Type | Sandwich ELISA |
| Kit Type | Colorimetric |
| Assay Time | 4.5 hours |
| Sample Type | serum, plasma |
| Assay Range | 62.5 - 4000 pg/ml |
| Sensitivity | 31.3 pg/ml |
| Specificity | This assay recognizes natural and recombinant human HE4 |
| Recovery | 90% to 113% |
| Precision | Inter-assay: 8.2%, Intra-assay: 4.5% |
| Dilution Factors | 1 to 2 with sample diluent |
| Gene ID | 10406 |
| Clinical indications | Serum HE4 is often measured together with CA125 to monitor progression of epithelial ovarian cancer after treatment. |
More info