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Human RRM2B ELISA Kit

NS-E10226

$576.00

Data sheet

Assay Range 47 - 3000 pg/ml

More info

Sensitivity
40 pg/mL
 
Size
96T
 
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
 
Assay Principle
Sandwich ELISA
 
Sample volume
100 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
 
 
 
 
Kit Components
 
 
 
1. Recombinant human RRM2B standard:6 ng/vial - 2.
 
 
2. One 96-well plate precoated with anti-human RRM2B Ab
 
 
3. Sample diluent buffer: 12 mL x 1
 
 
4. HRP conjugated anti-Human RRM2B Ab: 1 vial, dilution 1:1000.
 
 
5. Detection Antibody diluent buffer: 12 mL x 1
 
 
6. 20 x Wash Solution: 25 ml x 1.
 
 
7. TMB color developing agent A: 6 ml.
 
 
8. TMB color developing agent B: 6 ml.
 
 
9. TMB stop solution: 6ml.

 

Background

 

Ribonucleoside reductase subunit M2B (also known as RRM2B or p53R2) is an enzyme belonging to the iron- dependent ribonucleotide reductase (RNR) enzyme family, which is essential for DNA synthesis. It is widely expressed at a high level in skeletal muscle and at a weak level in thymus. RRM2B is also expressed in epithelial dysplasias and squamous cell carcinoma. This protein plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner. It translocates from cytoplasm to nucleus in response to DNA damage. Mutations in RRM2B gene have been shown to cause progressive external ophthalmoplegia. Increased expression of RRM2B has been correlated with gemcitabine resistance in human cholangiocarcinoma cells and may be predictive of lack of clinical benefit from gemcitabine for human cancers.  

 standard curve

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