Data sheet
| Assay Range | 15.6 - 1000 pg/ml |
More info
Sensitivity
15 pg/mL
Size
96T
Storage
Store at 2 - 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CIB2 standard:1 ng/vial - 2.
2. One 96-well plate precoated with anti-human CIB2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CIB2 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Calcium and integrin-binding family member 2, also known as Kinase-interacting protein 2, KIP-2 and CIB2, is a ubiquitously expressed protein. It is expressed in inner and outer segments of photoreceptor cells, as well as in the pigmented epithelium. In addition, CIB2 is observed in the inner and outer plexiform layers and in the ganglion cell layer. CIB2 is a calcium-binding protein, which is critical for proper photoreceptor cell maintenance and function. It may also play a role in calcium homeostasis and participate in calcium regulation in the mechano transduction process. CIB2 colocalized with ITGA7 at the myotendinous junctions (MTJ) and at the neuromuscular junctions.
