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T3 ELISA Kit

NR-R10074

$599.00

Data sheet

Assay Range 0-10ng/mL

More info

Sensitivity
0.2 ng/ml
 
Size
96T
 
Storage
Store at 2 - 8ºC. 
 
Assay Principle
Competitive ELISA
 
Sample volume
50 µL final volume, dilution factor varies on samples.
 
Detection Method
Chromogenic
   
Kit Components
 
 
 
1. Sandard set: 6 vials
 
 
2. One 96-well plate precoated with anti-T3 Antibody
 
 
3. 20 x Wash Solution: 25 ml x 1 
 
 
4. HRP conjugated T3: 1 vial, dilution 1:20 
 
 
5. T3-HRP conjugate diluent buffer: 12 mL x 1
 
 
6. TMB substrate: 12 ml x 1 
 
 
7. TMB stop solution: 12 mL x 1 
 
Background

 

Triiodothyronine, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growthanddevelopment, metabolism, bodytemperature, and heartrate. Triiodothyronine (T3) determination is an important factor in the diagnosis of hypothyroidism and hyperthyroidism. The level of T3 is normal in Euthyroid individuals. T3 level is decreased in hypothyroid cases and is increased in hyperthyroidism, Graves’ disease and pregnancy. Production of T3 and its prohormone thyroxine (T4) is activated by thyroid-stimulatinghormone (TSH), which is released from the pituitary gland. As concentrations of these hormones decrease, the pituitary gland increases production of TSH, and by these processes, a feedbackcontrolsystem is set up to regulate the amount of thyroid hormones that are in the bloodstream.

standard curve

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