Data sheet
| Validity | 12 ~ 18 Months from the Manufacturing Date |
| Size | 96T |
| Category | Parasitology ELISA kits |
| Background | Scrub typhus or Bush typhus is a form of typhus caused by the intracellular parasite Orientia tsutsugamushi, a Gram-negative α-proteobacterium of family Rickettsiaceae. Although the disease is similar in presentation to other forms of typhus, its pathogen is not anymore included in genus Rickettsia with the typhus bacteria proper, but in Orientia. The disease is thus frequently classified separately from the other typhi. Symptoms include fever, headache, muscle pain, cough, and gastrointestinal symptoms. More virulent strains of O. tsutsugamushi can cause hemorrhaging and intravascular coagulation. Morbilliform rash, eschar, splenomegaly and lymphadenopathies are typical signs. Pneumonitis, encephalitis, and myocarditis occur in the late phase of illness. With therapy, the fever will break within 36 hours, but if left untreated, complications or death may occur. |
| Assay principle | Wells of each plate on the Scrub Typhus IgG ELISA test have been coated with unique recombinant antigen mix. The serum sample is diluted in DAI sample diluent and applied to each well. After incubation and washing, the wells of this Scrub Typhus IgG ELISA Kit are treated with IgG enzyme conjugate (HRP). After a second incubation and washing step, TMB substrat is added. An acidic stopping solution is then added and the degree of enzymatic turnover of the substrate is determined by absorbance measurement at 450nm. The absorbance measured is directly proportional to the concentration of IgG antibodies to OT present on the S. typhus IgG ELISA kit. For additional details please refer to the instructions for use. |
| Assay Type | Indirect ELISA: Antigen Coated Plate |
| Kit Type | Colorimetric |
| Assay Time | 110 min |
| Sample Type | Serum or Plasma |
| Assay Range | Qualitative Positive; Negative control |
| Sample Volume | 50 ul |
More info
Kit Components
1. One 96-well plate precoated with Scrub Typhus
2. Assa diluent buffer: 12 mL x 1
3. HRP conjugated: 1 vial
4. Negative Control:1vial
5. Positive Control: 1 vial
6. 20 x Wash buffer: 1 vial
7. TMB substrat: 12 ml
8. TMB stop solution: 12 ml