| Specificity |
Antibody recognizes human, mouse, rat cardiac troponin I (cTnI) |
| Size |
100 µg |
| Storage |
2 - 8 ℃ |
| Application |
Western blot, ELISA, IHC |
| Immunogen/Antigen |
N-terminal region of human cTnI |
| Cross Reactivity |
human, mouse, rat |
| Isotype |
IgG1 |
| Reactivity |
only cardiac Troponin I, not skeletal muscle Troponin I |
| Host Species |
mouse |
| Supplied In |
PBS, pH 7.2, 0.02% NaN3 as a preservative |
| Dilution & Recommended Usage |
WB, 1:500; ELISA, 1:10:000. |
| Protein Background |
Troponin I (TnI), troponin T (TnT) and troponin C (TnC) form the troponin complex of the thin filaments of striated muscle. TnI is acts as the inhibitory subunit by blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains 3 genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. The TNNI3 gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in the TNNI3 gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). |
