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Data sheet
| Background | The FIA-CLIA Multiplex phospho-Akt1(Ser473) + Total Akt1 assay kit is used to measure both the phosphorylation of Akt1 and total levels of endogenous Akt1 in cellular lysates. The assay is an ideal system for the screening of modulators of receptor activation (e.g. agonists and antagonists) as well as agents acting intracellularly, such as small molecule inhibitors of signal transduction. The assay will measure Akt1 phosphorylation by either recombinant or endogenous receptors and can be applied to primary cells. |
| Assay principle | The FIA-CLIA Multiplex p-Akt 1/2/3 (Ser473) (HRP) + Total Akt1 (PE) assay kit is used to measure both phosphorylated and total levels of endogenous Akt in cellular lysates in a multiplexing Alpha no-wash assay. No specially engineered cell lines are required. The chemiluminescence signal corresponds to the phosphorylated Akt1 analysis, and the 575 nm (PE) signal corresponds to the total Akt1 analysis. This kit has been formulated to provide superior signal:background assay windows and to perform without interference in the presence of extraneous antibodies, making it amenable to the study of therapeutic and blocking antibodies. |
More info
- Few wash steps
- Suitable for both cell lysate and samples with colors
- Multiplexing assay
- Medium throughput
- Sensitive detection
- Less hands-on time than Western Blot
- Higher throughput than Western Blot
- Quantitative
- No interference from exogenous antibodies
- Results in less than 4 hours
Questions? Please contact us at info@novateinbio.com