IgG4 Food Antigen/Allergen Screen ELISA (88 Antigens)

 Microtiter Plate 1 microtiter plate, coated with 88 food antigens (see distribution scheme) and 8x reference antigen (1. strip, color coded violet). Ready-to-use.

 Standards A-F 6 x 0.5 mL, human plasma diluted with PBS/BSA, with 0.35, 0.70, 3.5, 17.5, 50 and 100 U/mL of IgG antibodies against egg white (f1). Addition of 0.05% sodium azide. Ready-to-use.

 Weak Positive Control 0.5 mL, human plasma diluted with PBS/BSA, including low concentrations of IgG antibodies. Addition of 0.05% sodium azide. Ready-to-use.

 Strong Positive Control 0.5 mL, human plasma diluted with PBS/BSA, including high concentrations of IgG antibodies. Addition of 0.05% sodium azide. Ready-to-use.

 Anti-human-IgG4 Enzyme Conjugate 15 mL, mouse-a-human-IgG4-AP, in proteinaceous buffer solution. Addition of 0.01% methyl

isothiazolone, 0.01% bromonitrodioxane and 5 mg/L ProclinTM. Ready-to-use.

 Substrate 15 mL, PNPP (Paranitrophenylphosphate). Ready-to-use.

 Stop Solution 15 mL, 1 M sodium hydroxide. Ready-to-use.

 Sample Diluent 40 mL, PBS/BSA buffer. Addition of 0.05% sodium azide. Ready-to-use.

 Washing Buffer 60 mL, PBS + Tween 20, 10x concentrate. Final concentration: dilute 1+9 with deionized water. If during the cold storage crystals precipitate, the concentrate should be warmed up at 37°C for 15 minutes.

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