Data sheet
| Assay Range | 0.78 – 50 pg/mL |
| Sensitivity | 0.25 pg/mL |
| Background | The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. The cell that initially produces IL-4, thus inducing Th2 differentiation, has not been identified, but recent studies suggest that basophils may be the effector cell.It is closely related and has functions similar to interleukin 13. Interleukin 4 has many biological roles, including the stimulation of activated B-cell and T-cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B-cell class switching to IgE, and up-regulates MHC class II production. IL-4 decreases the production of Th1 cells, macrophages, IFN-gamma, and dendritic cell IL-12. Overproduction of IL-4 is associated with allergies. Tissue macrophages play an important role in chronic inflammation and wound repair. The presence of IL-4 in extravascular tissues promotes alternative activation of macrophages into M2 cells and inhibits classical activation of macrophages into M1 cells. An increase in repair macrophages (M2) is coupled with secretion of IL-10 and TGF-β that result in a diminution of pathological inflammation. Release of arginase, proline, polyaminases and TGF-β by the activated M2 cell is tied with wound repair and fibrosis. |
| Sample | 5-10 µL mouse serum/plasma |
| Alternate Names | BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra |
| Gene ID | 16189 |
| UniProt ID | P07750 |
More info
Size
96T
Range
0.78 pg/mL-50 pg/mL
Sensitivity
0.24 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 °C for frequent use, at -20 °C for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
6 months at 4 °C and 12 months at -20 °C.
Application
serum, body fluids, tissue lysate, cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse IL-4 standard: 50 pg/tube-2.
2. One 96-well plate precoated with anti- mouse IL-4 antibody.
3. Sample diluent buffer: 12 ml x 2
4. Biotinylated anti- mouse IL-4 antibody: 60 µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Strepavidin-HRP: 300µL, dilution 1:40.
7. Strepavidin-HRPdiluent buffer: 12ml.
8. Chromogen-A, 6 ml; Chromogen-B, 6 ml.
9. Stop Solution: 6 ml.