Data sheet
| Assay Range | 0.78 – 50 pg/mL |
| Background | Interleukin 1α is responsible for the production of inflammation, as well as the promotion of fever and sepsis. IL-1α inhibitors are being developed to interrupt those processes and treat diseases. IL-1α is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum. The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. |
| Sample | 5-10 µL mouse serum/plasma |
| Alternate Names | IL1 alpha; IL-1 alpha; IL1; IL1A; IL-1A; IL1-ALPHA; IL1F1; IL-1F1; IL1F1hematopoietin-1; interleukin 1, alpha; interleukin-1 alpha; preinterleukin 1 alpha; pro-interleukin-1-alpha |
| Gene ID | 16175 |
| Citation | P01582 |
More info
Catalog No.
FM-HS100052
Size
96T
Range
0.78 pg/mL-50 pg/mL
Sensitivity
0.24 pg/mL
Specificity
No detectable cross-reactivity with any other cytokine.
Storage
Store at 4 °C for frequent use, at -20 °C for infrequent use.
Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Expiration
6 months at 4 °C and 12 months at -20 °C.
Application
serum, body fluids, tissue lysate, cell culture supernates.
Kit Components
1. Lyophilized recombinant mouse IL-1a standard: 50 pg/tube-2.
2. One 96-well plate precoated with anti- mouse IL-1a antibody.
3. Sample diluent buffer: 12 ml x 2
4. Biotinylated anti- mouse IL-1a antibody: 60 µL, dilution 1:180.
5. Antibody diluent buffer: 12ml.
6. Strepavidin-HRP: 300µL, dilution 1:40.
7. Strepavidin-HRPdiluent buffer: 12ml.
8. Chromogen-A, 6 ml; Chromogen-B, 6 ml.
9. Stop Solution: 6 ml.