| Assay principle |
This Japanese Encephalitis IgG ELISA Kit was based on standard sandwich enzyme-‐linked immunosorbent assay technology. Purified Japanese Encephalitis antigen and negative control antigen were precoated onto 96-‐ well plates, separately. Porcine Japanese Encephalitis antibodies, if presented in test samples, would bind to Japanese Encephalitis antigen precoated on the plate. Then HRP conjugated goat anti-‐porcine IgG antibody was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Japanese Encephalitis IgG amount of sample captured in plate. |
