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Erythropoietin-Alpha Human Recombinant ( EPO a Human )
DescriptionErythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and
Data sheet
| Formulation | Each mg of lyophilized powder contains 0.59 mg sodium citrate, 0.58 mg sodium chloride and 0.006 mg citric acid. |
| Solubility | It is recommended to reconstitute the lyophilized Epoetin-a in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Inactivation | Protein quantitation was carried out by two independent methods:1. UV spectroscopy at 280 nm using the absorbency value of 0.885 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of FGF2 as a Reference Standard. |
| Description | Erythropoietin-alpha Human Recombinant is produced in Chinese hamster ovary (CHO) cells by recombinant DNA technology is a single, polypeptide chain containing 166 amino acids and having a predicted molecular mass of 21,000 Dalton and apparent glycosylated molecular mass of 36-40kDa. EPO-a is purified by proprietary chromatographic techniques. |
| Protein Background | This gene is a member of the EPO/TPO family and encodes a secreted, glycosylated cytokine composed of four alpha helical bundles. The protein is found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. This protein also has neuroprotective activity against a variety of potential brain injuries and antiapoptotic functions in several tissue types. |
| Expression host | Chinese Hamster Ovary Cells(CHO). |
| Synonyms | Erythropoietin-Alpha, EPO-a, EPO-alpha, Epoetin, EP, MGC138142. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Erythropoietin-a although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution EPO-alpha should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | APPRLICDSR VLERYLLEAK EAENITTGCA EHCSLNENIT VPDTKVNFYA WKRMEVGQQA VEVWQGLALL SEAVLRGQAL LVNSSQPWEP LQLHVDKAVS GLRSLTTLLR ALGAQKEAIS PPDAASAAPL RTITADTFRK LFRVYSNFLR GKLKLYTGEA CRTGDR. |
| Biological Activity | The Specific Activity was measured by Normocyth -aemic mice and was found to be 150,000 IU/mg. |
| References | 1.Title:Zinc Transporters ZnT1 (Slc30a1), Zip8 (Slc39a8), and Zip10 (Slc39a10) in Mouse Red Blood Cells Are Differentially Regulated during Erythroid Development and by Dietary Zinc Deficiency13Publication:Journal of Nutrition, doi:10.3945/jn.108.093575Vol. 138, No. 11, 2076-2083, November 2008© 2008 American Society for Nutrition J. Nutr. 138:2076-2083, November 2008Link:http://jn.nutrition.org/content/138/11/2076.full2.Title:Systemically delivered Erythropoietin transiently enhances adult hippocampal neurogenesis.Publication:Article first published online: 7 MAY 2007 DOI:10.1111/j.1471-4159.2007.04684.x Journal of Neurochemistry Volume 102, Issue 6, pages 19531965, September 2007.Link:http://onlinelibrary.wiley.com/doi/10.1111/j.1471-4159.2007.04684.x/full3.Title:ZINC TRANSPORTER EXPRESSION IN MATURE RED BLOOD CELLS ANDDIFFERENTIATING ERYTHROID PROGENITOR CELLS.Publication:UNIVERSITY OF FLORIDA© 2007 Moon-Suhn RyuLink:http://ufdcimages.uflib.ufl.edu/UF/E0/02/14/46/00001/ryu_m.pdf4.Title:Cell Therapy with Human Renal Cell Cultures Containing Erythropoietin-Positive Cells Improves Chronic Kidney Injury.Publication:First Published Online May 3, 2012 doi: 10.5966/sctm.2011-0048 Stem Cells Trans Med May 2012 vol. 1 no. 5 373-383Link:http://stemcellstm.alphamedpress.org/content/1/5/373.full |