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Fibroblast Growth Factor-Basic Human Recombinant ( FGF 2 Human )
DescriptionFibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 155 amino acids and having a molecular mass of 17353 Dalton.The FGF-b is purified by proprietary
Data sheet
| Formulation | The protein was lyophilized from a concentrated (1mg/ml) solution in PBS, pH 7.4. |
| Solubility | It is recommended to reconstitute the lyophilized Fibroblast Growth Factor Basic in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Purity | Greater than 98.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Description | Fibroblast Growth Factor-2 Human Recombinant (FGF-2) produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 154 amino acids and having a molecular mass of 17.2kDa.The FGF-b is purified by proprietary chromatographic techniques. |
| Protein Background | Basic fibroblast growth factor is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. |
| Expression host | Escherichia Coli. |
| Synonyms | Prostatropin, HBGH-2, HBGF-2, FGF-2, FGF-b. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Stability | Lyophilized Fibroblast Growth Factor-2 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF-b should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Amino acid sequence | AAGSITTLPA LPEDGGSGAF PPGHFKDPKR LYCKNGGFFL RIHPDGRVDG VREKSDPHIK LQLQAEERGV VSIKGVCANR YLAMKEDGRL LASKCVTDEC FFFERLESNN YNTYRSRKYT SWYVALKRTG QYKLGSKTGP GQKAILFLPM SAKS. |
| Biological Activity | The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is |
| References | 1.Title:Activation of the Hedgehog pathway in pilocytic astrocytomasPublication:Neuro Oncol (2010) 12 (8): 790-798. doi: 10.1093/neuonc/noq026 First published online: March 11, 2010 Link:http://neurooncology.oxfordjournals.org/content/12/8/790.full2.Title:Phenotype reversion in fetal human liver epithelial cells identifies the role of an intermediate meso-endodermal stage before hepatic maturation Publication:Advance Online Publication March 4, 2008 doi: 10.1242/?jcs.019315 April 1, 2008 J Cell Sci 121, 1002-1013. Link:http://jcs.biologists.org/content/121/7/1002.full3. Title:Embryonic Stem Cell-Derived Glial Precursors as a Vehicle for Sulfamidase Production in the MPS-IIIA Mouse Brain .Publication: Cell Transplantation ISSN: 0963-6897 DOI: 10.3727/096368910X498944 Volume 19, Issue 8, pages 985-998 Copyright © 2010 Cognizant Comm. CorpLink:http://www.ingentaconnect.com/content/cog/ct/2010/00000019/00000008/art000074.Title:Adult Human Dental Pulp Stem Cells Differentiate Toward Functionally Active Neurons Under Appropriate Environmental Cues.Publication: Article first published online: 22 MAY 2008 DOI:10.1634/stemcells.2007-0979 Copyright © 2008 AlphaMed Press.Link:http://onlinelibrary.wiley.com/doi/10.1634/stemcells.2007-0979/full5.Title: Successful implantation of physiologically functional bioengineered mouse internal anal sphincterPublication: American Journal of Physiology-Gastrointestinal and Liver Physiology 299.2 (2010): G430-G439.Link: http://ajpgi.physiology.org/content/299/2/G430 |