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Macrophage Migration Inhibitory Factor Human Recombinant ( MIF Human )
DescriptionMIF human Recombinant was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated,
Data sheet
| Formulation | 1mg/ml solution containing 50mM Tris-HCl pH-8, 0.5mM DTT & 10% glycerol. |
| Purity | Greater than 98.0% as determined by SDS-PAGE. |
| Description | MIF human Recombinant was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.Macrophage Inducing Factor Human Recombinant is a single, non-glycosylated, polypeptide chain containing 115 amino acids and having a molecular mass of 12 kDa. |
| Protein Background | The cytokine Macrophage migration inhibitory factor (MIF) has been identified to be secreted by the pituitary gland and the monocyte/macrophage and to play an important role in endotoxic shock. MIF has the unique property of being released from macrophages and T cells in response to physiological concentrations of glucocorticoids. The secretion of MIF is tightly regulated and decreases at high, anti-inflammatory steroid concentration. |
| Expression host | Escherichia Coli. |
| Synonyms | Phenylpyruvate tautomerase, Glycosylation-inhibiting factor, GIF, MMIF, MIF. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MPMFIVNTNV PRASVPDGFL SELTQQLAQA TGKPPQYIAV HVVPDQLMAF GGSSEPCALC SLHSIGKIGG AQNRSYSKLL CGLLAERLRI SPDRVYINYY DMNAANVGWN NSTFA. |
| References | Title: Macrophage migration inhibitory factor regulates proliferation of gastric cancer cells via the PI3K/Akt pathway Publication: WJG 15.44 (2009): 5541. Link: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2785056/ |