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Soybean agglutinin,SBA ELISA Kit
Soybean agglutinin,SBA ELISA Kit For research use only. Not for use in diagnostic procedures.For general protocol and instruction, please click the following links:Quantitative Elisa Kit InstructionSandwich ELISA kit general instruction Competition
Data sheet
| Storage | 2 - 8 ℃ |
| Background | Soybean agglutinins (SBA) also known as soy bean lectins (SBL) are lectins found in soybeans. It is a family of similar legume lectins. As a lectin, it is an antinutrient that chelates minerals. In human foodstuffs, less than half of this lectin is deactivated even with extensive cooking (boiling for 20 minutes). SBAs have a molecular weight of 120 kDa and an isoelectric point near pH 6.0. SBA preferentially binds to oligosaccharide structures with terminal α-helix or β-sheet linked N-acetylgalactosamine, and to a lesser extent, galactose residues. Binding can be blocked by substitutions on penultimate sugars, such as fucose attached to the penultimate galactose in blood group B. Soybean lectin has a metal binding site, which is conserved among beans. SBA binds to intestinal epithelial cells, causing inflammation and intestinal permeability, and is a major factor in acute inflammation from raw soybean meal fed to animals. An important application for SBA is the separation of pluripotent stem cells from human bone marrow. Cells fractionated by SBA do not produce graft vs host disease and can be used in bone marrow transplantation across histocompatibility barriers. SBA binding has been investigated as a useful tool for detection of stomach cancer. |
| Alternate Names | soybean agglutinin, SBA, soybean lectin, SBL |
| Assay Type | Competitive ELISA |
| Kit Type | Colorimetric |
| Assay Time | 2.5 hours |
| Sample Type | solubilized powder, serum/plasma, extract, cell lysate |
| Assay Range | 4.9 - 20,000 ng/ml |
| Sensitivity | 1.9 ng/ml |
| Specificity | only recognizes soybean agglutinin, not other lectins. |
More info
Soybean agglutinin,SBA ELISA Kit
For protocols and availability questions, please fill out our contact us form and make sure to include your company name, business email, and phone number. We will contact you within 24 hours.
Different lot may vary, please follow the instruction coming with the kit.
PRODUCT CITATIONS
1.Validation of a Method for Quantitation of Soybean Lectin in Commercial Varieties
Matthew L. Breeze, Elisa Leyva-Guerrero, Grant R. Yeaman, Yelena Dudin, Ryan Akel, Phil Brune, Fred Claussen, Cecil Dharmasri, Jenny Golbach, Rong Guo, Carl Maxwell, Laura Privalle, Hilary Rogers, Kai Liu, Guomin Shan, Michele Yarnall, Denise Thiede, Nancy Gillikin
JAOCS, 2015, 92 (8), pp 1085-10922.
2. ECL Biosensor for Sensitive Detection of Soybean Agglutinin Based on AuPt@C 60 Nanoflowers Enhanced N-(aminobutyl)-N-(ethylisoluminol), Liu D et al, Journal of The Electrochemical Society, 10.1149/2.0541902jes, 166, 2, (B49-B55), (2019).