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Data sheet
| Formulation | PSME3 protein solution (0.5mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 200mM NaCl, 2mM DTT and 40% glycerol. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Description | PSME3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 274 amino acids (1-254 a.a.) and having a molecular mass of 31.7kDa. PSME3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Protein Background | PSME3 is a member of the PA28 family. The 26S proteasome is an extremely organized multicatalytic proteinase complex composed of 2 complexes, a 20S core and a 19S regulator. Proteasomes are spread all over the eukaryotic cells in large quantities and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSME3 stimulates the trypsin-like catalytic subunit of the proteasome and inhibits the chymotrypsin-like and postglutamyl-preferring (PGPH) subunits. PSEM3 enables the MDM2-p53/TP53 collaboration that encourages ubiquitination- and MDM2-dependent proteasomal degradation of p53/TP53, restricting its growth and causing inhibited apoptosis after DNA damage. |
| Expression host | E.coli. |
| Synonyms | Proteasome (prosome, macropain) activator subunit 3 (PA28 gamma; Ki), PA28G, Ki, Ki nuclear autoantigen, PA28-gamma, REG-GAMMA, Activator of multicatalytic protease subunit 3, Proteasome activator 28 subunit gamma, 11S regulator complex subunit gamma, PA28gamma, |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MASLLKVDQE VKLKVDSFRE RITSEAEDLV ANFFPKKLLE LDSFLKEPIL NIHDLTQIHS DMNLPVPDPI LLTNSHDGLD GPTYKKRRLD ECEEAFQGTK VFVMPNGMLK SNQQLVDIIE KVKPEIRLLI EKCNTVKMWV QLLIPRIEDG NNFGVSIQEE TVAELRTVES EAASYLDQIS RYYITRAKLV SKIAKYPHVE DYRRTVTEID EKEYISLRLI ISELRNQYVT LHDMILKNIE KIKRPRSSNA ETLY |
More info
, 11S regulator complex subunit gamma, Activator of multicatalytic protease subunit 3, Ki, Ki nuclear autoantigen, macropain) activator subunit 3 (PA28 gamma; Ki), PA28G, PA28gamma, PA28-gamma, Proteasome activator 28 subunit gamma, REG-GAMMA, Proteasome (prosome